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2002 - Cleyton Eduardo Mendes de Toledo

MSc. Thesis

 

Author: Cleyton Eduardo Mendes de Toledo

Postgraduate Program in Pharmaceutical Sciences

Date of Defense: October 11, 2002

Advisor: Prof. Dr.  João Carlos Palazzo de Mello

Examination Board:  Prof. Dra. Raquel Regina Duarte Moreira

                               Prof. Dr. Pedro Ros Petrovick

 

Title: STUDIES ANATOMICAL, BIOLOGICAL ANDCHEMICAL PEEL AND EXTRACTS OBTAINEDBARBATIMÃO [Stryphnodendron adstringens (Mart.) Coville, Leguminosae

Abstract: The stem barks of Stryphnodendron adstringens (Mart.) Coville is at a long time used by the native population for treatment of several illnesses, mainly as healing and antiseptic. This work analysed chemically, through biological screening, obtaining fractions and substances starting from the crude extract of its stem bark, besides testing them for the antibacterial and leishmanicidal activities. Carry also an analysis anatomic to aim to know the structures of the barks. Of the 12 subfractions obtained starting from the ethylacetate fraction were isolated and identified 6 substances: gallocatechin, epigallocatechin, 4’-O-methylgallocatechin, 4’-O-methylgallocatechin-(4a→8)-4’-O-methylgallocatechin, 4’-O-methyl-robenitinidin-(4α→8)-4’-O-methyl-epigallocatechin and 4’-O-methyl-robenitinidin-(4α→8)-4’-O-methyl-gallocatechin. The 12 subfractions were tested for antibacterial activity against Staphylococcus aureusBacillus subtilis and Pseudomonas aeruginosa. All fractions presented activity against the first two bacterias, while only F3#12 it showed action against P. aeruginosa. The subfractions F3#3, F3#5, F3#7, F3#9 and F3#12 were tested against Leishmania (L.) amazonensis. All fractions presented activity, being the two last the most effective in smaller concentrations. Thus, it is ended that the anatomical curt made possible parameters for the quality control of the vegetable, and their extracts and fractions were shown effective in the biological tests and with a rich chemical content in flavan-3-ols and dimers of condensed tannins.

Keywords: Stryphnodendron adstringens; Condensed tannins; Leguminosae; Antibacterian activity; Leishmanicidal activity.

 

Dissertation PDF: Cleyton Eduardo Mendes de Toledo

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