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2004 - Andréia Cristina Conegero Sanches

MSc. Thesis

Author: Andréia Cristina Conegero Sanches

Postgraduate Program in Pharmaceutical Sciences - State University of Maringá

Area of Knowledge: Pharmacognosy

Date of Defense: January 23, 2004

Advisor: Prof. Dr.  João Carlos Palazzo de Mello

Examination Board:  Prof. Dr. Celso Vataru Nakamura

  Prof. Dr. Luis Vitor Silva do Sacramento

 

Title: Pharmacognostic study of shells Stryphnodendron obovatum Benth., Antioxidant activity, antimicrobial and healing action of its extracts

Abstract: “Barbatimão de folha miúda” Stryphnodendron obovatum Benth. was used popularly as: antimicrobial, in wound healing, leukorrhea, blenorrhoea, diarrhoea, and anti-inflamatory. The present work was to accomplish study pharmacognostic and to check some activities of EB, fractions and subfractions: antioxidant, antimicrobial and wound healing properties. It was possible to trace a profile of the representatives chemists of the vegetable drug, and also to observ that the best time for obtaining tannins was the Spring. From CC Sephadexâ LH-20 it 22 subfractions were obtained using FAcOEt, and 5 compounds were isolated and identified: epigallocatechin, gallocatechin, epigallocatechin-(4b®8)-gallocatechin, epigallocatechin-(4b®8)-epigallocatechin and robinetinidol-(4b®8)-epigallocatechin. The study of the antioxidant activity showed that the sufractions 6 to 12 demonstrated antioxidant activity as compared to the controls quercetin, rutin, galic acid, and astilbin. All the fractions, subfractions and extracts were tested against  the microorganisms Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida parapsilosis, Candida krusei, Candida tropicalis. The subfractions F#13 to F#22 were active against Gram-positive microorganisms, C. albicans and C. parapsilosis. FEB and FAcOEt increased the metafase number/area indicanting improvement in the proliferation cellular in the wounds using pomade at 2.5%.

Keywords: Stryphnodendron obovatum, Leguminosae, quality control, tannin, antioxidant activity, antimicrobial activity, wound healing properties.

 

Dissertation PDF: Andreia Cristina Conegero Sanches

 

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