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2008 - Luciana Grandis Wolf

MSc. Thesis

Author: Luciana Grandis Wolf

Postgraduate Program in Pharmaceutical Sciences - State University of Maringá

Area of Knowledge: Pharmacognosy

Date of Defense: August 29, 2008

Advisor: Prof. Dr.  João Carlos Palazzo de Mello

Examination Board:  Prof. Dra. Mara Lane Carvalho Cardoso

                             Prof. Dra. Marli Miriam de Souza Lima


Title: Assessment of the physico-chemical quality of herbal drugs

Abstract: The use of phytomedicines is an ancient practice, but the increase in the marketing of this kind of medicine increased the requirement of quality, thus ensuring the safety and efficacy of medicine. The quality must be achieved through the raw materials control, the finished product, packaging materials and pharmaceutical formulation. One of the goals of this study was to evaluate the quality of fifteen plant species through physicalchemical and chromatography analysis. Phytochemical profile was evaluated by preliminary analysis and was performed the determination of extractives (DE), the loss on drying (LD) and the total ashes (TA). In the chromatographic analysis by thinlayer chromatography was determined the mobile phase, the characteristic of the substance of reference and the revealing system. The plant species examined were: Persea gratissima (DE=22,33%±0,83; LD=9,47%±0,15; TA=5,19%±0,17; Rf=0,40 for quercetin), Schinus terebenthifolius (DE=25,86%±0,24; LD=12;93%±0,48; TA=4,21%±0,11; Rf=0,81 for catechin e Rf=0,19 for gallic acid), Stryphnodendron adstringens (DE=39,6%±1,01; LD=11,23%±0,45; TA=3,32%±0,11; Rf=0,79 for 4’-Ometylgallocatechin), Arctium lappa (DE=33,94%±1,32; LD=11,45%±0,37; TA=3,42%±0,10; Rf=0,58 for clorogenic acid and Rf=0,67 for rutin), Baccharis trimera (DE=22,24%±0,82; LD=10,86%±0,20; TA=6,46%±0,19; Rf=0,35 for caffei cacid and Rf=0,40 for quercetin), Aesculus hippocastanum (DE=61,23%±1,82; LD=9,89%±0,21; TA=2,82%±0,07; Rf=0,61 for escin), Equisetum arvense (DE=18,09%±0,42; LD=11,11%±0,13; TA=19,03%±0,19; Rf=0,35 for caffeic acid), Centella asiatica (DE=29,78%±1,32; LD=10,54%±0,45; TA=17,31%±0,63; Rf=0,40 for quercetin), Camellia sinensis (DE=42,39%±0,75; LD=8,21%±0,21; TA=3,12%±0,14; Rf=0,58 for caffeine, Rf=0,80 for epicatechin and Rf=0,66 for epicatechin-3-O-gallate), Crataegus oxyacantha (DE=35,68%±0,9; LD=9,30%±0,15; TA=9,56%±0,13; Rf=0,35 for caffeic acid and Rf=0,58 for clorogênico acid), Echinacea purpurea (DE=30,24%±0,34; LD=11,61%±0,36; TA=9,56%±0,10; Rf=0,35 for caffeic acid), Harpagophytum procumbens (DE=75,12%±1,14; LD=10,5%±0,27; TA=4,64%±0,14; Rf=0,72 for harpagoside), Paullinia cupana (DE=44,86%±0,60; LD=10,40%±0,14; TA=2,18%±0,05; Rf=0,58 for caffeine and Rf=0,81 for catechin), Heteropteris aphrodisiaca (DE=33,41%±1,05; LD=11,95%±0,35; TA=4,73%±0,07; Rf=0,73 for astilbin), Curcuma zedoaria (DE=44,61%±0,63; LD=12,59%±0,08; TA=14,50%±0,26; Rf=0,60 for curcumin).

Keywords:  chromatographic analysis; drug plants; quality control; thin layer chromatography.


Dissertation PDFLuciana Grandis Wolf

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