You are here: Home / Publications / Dissertations / 2008 - Renato Braz

2008 - Renato Braz

MSc. Thesis

Author: Renato Braz

Postgraduate Program in Pharmaceutical Sciences - State University of Maringá

Area of Knowledge: Pharmacognosy

Date of Defense: August 29, 2008

Advisor: Prof. Dr.  João Carlos Palazzo de Mello

Examination Board:Prof. Dr. Marcos Luciano Bruschi

                            Profa. Dra. Andrea Diniz

 

Title: Contribution to protocol of quality control medicinal plants

Abstract: Nowadays people have been looking for in the products natural healthier life habits, from the feeding to the therapy medicines. This trend associated to the high cost of the synthetic medicines and high incidence of adverse reactions has been conduced to progressive increase in the production and using of drug plants and natural medicines. Thus, care about quality of these products should also increase, since that incidence of contaminations, falsifications and adulterations are frequent. Consequently, these factors make difficult the safe practice of the phytotherapy due several factors, as the difficulty in the correct identification of the botanical material and the small amount or inexistence of studies of safety, effectiveness and quality of great number of the used plants. The maintenance and the warranty of the quality of these products involve a series of experiments that constitute the quality control, in which physical- chemistry analyses are inserted and by this are suggested in the present work. They are: preliminary phytochemical analysis, loss on drying (LD), determination of extractives (DE), total ashes (TA) and the chromatography analysis. Analyzed plant species were: Cinara scolymus (LD= 11.28% ± 0.29; DE= 35.42% ± 1.07; TA = 13.72% ± 1.89; Rf = 0.35 for cafeic acid and 0.58 for clorogenic acid), Peumus boldus (LD= 10.42% ± 0.30; DE= 31.36% ± 0.65; TA = 14.5% ± 0.26; Rf = 0.51 for boldine and 0.59 for yohimbine), Trichilia catigua (LD= 10.42% 0.08±; DE= 24.62% ± 0.74; TA = 6.90% ± 0.06; Rf = 0.58 for cinchonain), Maytenus ilicifolia (LD= 10.66% ± 0.28; DE= 30.26% ± 0.79; TA = 8.12% ± 0.14; Rf = 0.77 for epicatechin), Foeniculum vulgare (LD= 18.18% ± 0.37; DE= 24.01% ± 0.46; TA = 9.15% ± 0.29; Rf = 0.35 for cafeic acid), Mikania glomerata (LD= 10.57% ± 0.24; DE= 24.43% ± 0.60; TA = 5.40% ± 0.11; Rf = 0.79 for cumarin and 0.39 for o-cumaric acid), Hamamelis virginiana (LD= 6.6% ± 0.13; DE=% ±; TA = 4.75% ± 0.97; Rf = 0.18 for gallic acid), Hipericum perforatum (LD= 11.96% ± 0.24; DE= 23.31% ± 0.66; TA = 3.33% ± 0.08; Rf = 0.20 for hyperoside), Croton moritibensis (LD= 10.25% ± 0.08; DE= 2.29% ± 0.08; TA = 2.09% ± 0.07; Rf = 0.76 for lupeol), Achyrocline satureioides (LD= 10.02% ± 0.23; DE= 14.16% ± 0.25; TA = 3.17% ± 0.08; Rf = 0.35 for cafeic acid and 0.40 for quercetin), Passiflora alata (LD= 10.50% ± 0.25; DE= 21.02% ± 0.48; TA = 7.98% ± 0.16; Rf = 0.35 for cafeicacid and 040 for quercetin), Sambucus nigra (LD= 14.73% ± 0.34; DE= 29.69% ± 0.22; TA = 5.67% ± 0.15; Rf = 0.35 for cafeic acid and 0.36 for rutin), Plantago major (LD= 9.87% ± 0.19; DE= 42.70% ± 0.77; TA = 13.70% ± 0.37; Rf = 0.35 for cafeic acid and 0.58 for clorogenic acid), Arctostaphylos grape-ursi (LD= 9.49% ± 0.29; DE= 38.35% ± 0.78; TA = 3.23% ± 0.09; Rf= 0.49 for hydroquinone and 0.21 for gallic acid).

Keywords: quality control, physical-chemistry analyses, thin layer chromatography, drug plants

 

Dissertation PDF: Renato Braz

                          Catalyst Sheet

                          Approval Term

 

WARNING – THE PERMISSION TO ACCESS THIS WORK IS SUBJECT TO THE ACCEPTANCE OF THE FOLLOWING TERMS OF USE:  This work is only for private use of research and teaching activities. Is unauthorized reproduction for any profit. This reservation of rights covers all document data as well as its content. Use or quote parts of the document must bear the name of the person author of the work.