2017 - André Oliveira Fernandes da Silva

MSc. Thesis

Author: André Oliveira Fernandes da Silva

Postgraduate Program in Pharmaceutical Sciences - State University of Maringá

Area of Knowledge: Pharmacognosy

Date of Defense: July 31, 2017

Advisor: Prof. Dr.  João Carlos Palazzo de Mello

Examination Board:  Prof. Dra. Mara Lane Carvalho Cardoso

                               Prof. Dr. Cláudio Roberto Novello

Title: Isolation and identification of polyphenols in extract of Catuaba (Trichilia catigua).

Abstract: Trichilia catigua A. Juss. (Meliaceae), known as catuaba and catiguá, is a plant widely found in South America to Central America. The barks are mainly used as stimulants; and have antioxidant, analgesic, vasodilator, anti-inflammatory and anti-depressant action, and is also used in folk medicine as a tonic for the treatment of fatigue, stress, impotence and memory deficits. At the chemical point, the species stands out for having a high content of phenolic compounds, including flavonoids and tannins. Considering the scientific and economic interest that this kind awakens, it is essential to conduct studies aimed at the isolation and structural identification of substances present in the extract. In this study, semipurified fraction obtained from the crude extract acetone:water from T. catigua barks was subjected to various chromatographic methods, such as column chromatography, high speed countercurrent (MLCCC), and thin-layer chromatography with the aim of isolate phenolic substances. Identification was performed by spectroscopic methods of nuclear magnetic resonance (NMR) 1D (1H and 13C), 2D NMR (1H-1H-COSY), and mass spectrometry. According the isolation, it was identified the following compounds: neoclorogenic acid, epicatechin, and cinchonains IA, IB and IIB.

Keywords: Trichilia catigua, polyphenols, chromatographic methods, neoclorogenic acid, cinchonains IA, IB and IIB.

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