2002 - Cleyton Eduardo Mendes de Toledo
MSc. Thesis
Author: Cleyton Eduardo Mendes de Toledo
Postgraduate Program in Pharmaceutical Sciences
Date of Defense: October 11, 2002
Advisor: Prof. Dr. João Carlos Palazzo de Mello
Examination Board: Prof. Dra. Raquel Regina Duarte Moreira
Prof. Dr. Pedro Ros Petrovick
Title: STUDIES ANATOMICAL, BIOLOGICAL ANDCHEMICAL PEEL AND EXTRACTS OBTAINEDBARBATIMÃO [Stryphnodendron adstringens (Mart.) Coville, Leguminosae
Abstract: The stem barks of Stryphnodendron adstringens (Mart.) Coville is at a long time used by the native population for treatment of several illnesses, mainly as healing and antiseptic. This work analysed chemically, through biological screening, obtaining fractions and substances starting from the crude extract of its stem bark, besides testing them for the antibacterial and leishmanicidal activities. Carry also an analysis anatomic to aim to know the structures of the barks. Of the 12 subfractions obtained starting from the ethylacetate fraction were isolated and identified 6 substances: gallocatechin, epigallocatechin, 4’-O-methylgallocatechin, 4’-O-methylgallocatechin-(4a→8)-4’-O-methylgallocatechin, 4’-O-methyl-robenitinidin-(4α→8)-4’-O-methyl-epigallocatechin and 4’-O-methyl-robenitinidin-(4α→8)-4’-O-methyl-gallocatechin. The 12 subfractions were tested for antibacterial activity against Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. All fractions presented activity against the first two bacterias, while only F3#12 it showed action against P. aeruginosa. The subfractions F3#3, F3#5, F3#7, F3#9 and F3#12 were tested against Leishmania (L.) amazonensis. All fractions presented activity, being the two last the most effective in smaller concentrations. Thus, it is ended that the anatomical curt made possible parameters for the quality control of the vegetable, and their extracts and fractions were shown effective in the biological tests and with a rich chemical content in flavan-3-ols and dimers of condensed tannins.
Keywords: Stryphnodendron adstringens; Condensed tannins; Leguminosae; Antibacterian activity; Leishmanicidal activity.
Dissertation PDF: Cleyton Eduardo Mendes de Toledo
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