2002 - Karen Janaína Galina
MSc. Thesis
Author: Karen Janaína Galina
Postgraduate Program in Pharmaceutical Sciences-Pharmaceutical and Pharmaceutical Development of the Paulista State University "Júlio de Mesquita Filho"
Date of Defense: January 23, 2003
Advisor: Prof. Dr. João Carlos Palazzo de Mello
Examination Board: Prof. Dra.Hérida Regina Nunes Marona
Title: Guazuma ulmifolia Lam., Sterculiaceae: botanical, chemical, and microbiological study
Abstract: Guazuma ulmifolia Lam., Sterculiaceae, popularly known as “mutamba”, presents wide geographical distribution, ranging from Mexico to Southern Brazil. The interest in the microbial and chemical study is due to its use for treatment for hair loss. This activity can be attributed to tannins that have many pharmacological activities such anti-inflammatory, anti-oxidant, radical scavenging, anti-ulcer, anti-microbial, anti-viral and capillary protective action. The material was collected in the field vicinity of Londrina, Paraná state and, in the field vicinity of Guariroba, São Paulo state, and, after botanical analyses, the identification confirmed the material being Guazuma ulmifoliaLam., var. tomentella. In the microscopic analyses, big secretories ducts among the cells of the parenquimal tissue and star-shaped trichoms on the epidermis were detected. In the chemical analyses was carried out the separation of compounds from the crude extract by a column chromatography on Sephadex® LH-20. By the biologic point of view, observed the in vitro anti-bacterial activity from crude and semi-purified extracts, by microdilution method. The result for activity against Staphyllococcus aureus and Bacillus subtilis was showed by the aqueous fraction of crude extract (MIC 31.25 mg/ml, and MBC 250 mg/ml; MIC 125 mg/ml and MBC 500 mg/ml, respectively). Moreover, the ethyl acetate fraction presented some activity against Pseudomonas aeruginosa (MIC 250 mg/ml). Phytochemical approach proved the presence of tannins, flavonoids and saponins. From the fractionation of a crude extract of G. ulmifoliastem bark, with the ethyl acetate fraction, led to the isolation of one flavan-3-ol (epicatechin) and two dimmers of condensed tannin [procyanidin B2 and epiafzelechin-(4b®8)-epicatechin], whole characterized by spectroscopic methods by NMR, MS and comparison with literature values.
Keywords: Guazuma ulmifolia; Sterculiaceae; condensed tannins; botanical analyses, antibacterial activity.
Dissertation PDF: Karen Janaina Galina
WARNING – THE PERMISSION TO ACCESS THIS WORK IS SUBJECT TO THE ACCEPTANCE OF THE FOLLOWING TERMS OF USE:
This work is only for private use of research and teaching activities. Is unauthorized reproduction for any profit. This reservation of rights covers all document data as well as its content. Use or quote parts of the document must bear the name of the person author of the work.
