2004 - Andréia Cristina Conegero Sanches
MSc. Thesis
Author: Andréia Cristina Conegero Sanches
Postgraduate Program in Pharmaceutical Sciences - State University of Maringá
Area of Knowledge: Pharmacognosy
Date of Defense: January 23, 2004
Advisor: Prof. Dr. João Carlos Palazzo de Mello
Examination Board: Prof. Dr. Celso Vataru Nakamura
Prof. Dr. Luis Vitor Silva do Sacramento
Title: Pharmacognostic study of shells Stryphnodendron obovatum Benth., Antioxidant activity, antimicrobial and healing action of its extracts
Abstract: “Barbatimão de folha miúda” Stryphnodendron obovatum Benth. was used popularly as: antimicrobial, in wound healing, leukorrhea, blenorrhoea, diarrhoea, and anti-inflamatory. The present work was to accomplish study pharmacognostic and to check some activities of EB, fractions and subfractions: antioxidant, antimicrobial and wound healing properties. It was possible to trace a profile of the representatives chemists of the vegetable drug, and also to observ that the best time for obtaining tannins was the Spring. From CC Sephadexâ LH-20 it 22 subfractions were obtained using FAcOEt, and 5 compounds were isolated and identified: epigallocatechin, gallocatechin, epigallocatechin-(4b®8)-gallocatechin, epigallocatechin-(4b®8)-epigallocatechin and robinetinidol-(4b®8)-epigallocatechin. The study of the antioxidant activity showed that the sufractions 6 to 12 demonstrated antioxidant activity as compared to the controls quercetin, rutin, galic acid, and astilbin. All the fractions, subfractions and extracts were tested against the microorganisms Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida parapsilosis, Candida krusei, Candida tropicalis. The subfractions F#13 to F#22 were active against Gram-positive microorganisms, C. albicans and C. parapsilosis. FEB and FAcOEt increased the metafase number/area indicanting improvement in the proliferation cellular in the wounds using pomade at 2.5%.
Keywords: Stryphnodendron obovatum, Leguminosae, quality control, tannin, antioxidant activity, antimicrobial activity, wound healing properties.
Dissertation PDF: Andreia Cristina Conegero Sanches
WARNING – THE PERMISSION TO ACCESS THIS WORK IS SUBJECT TO THE ACCEPTANCE OF THE FOLLOWING TERMS OF USE:
This work is only for private use of research and teaching activities. Is unauthorized reproduction for any profit. This reservation of rights covers all document data as well as its content. Use or quote parts of the document must bear the name of the person author of the work.
