2008 - Renato Braz
MSc. Thesis
Author: Renato Braz
Postgraduate Program in Pharmaceutical Sciences - State University of Maringá
Area of Knowledge: Pharmacognosy
Date of Defense: August 29, 2008
Advisor: Prof. Dr. João Carlos Palazzo de Mello
Examination Board:Prof. Dr. Marcos Luciano Bruschi
Profa. Dra. Andrea Diniz
Title: Contribution to protocol of quality control medicinal plants
Abstract: Nowadays people have been looking for in the products natural healthier life habits, from the feeding to the therapy medicines. This trend associated to the high cost of the synthetic medicines and high incidence of adverse reactions has been conduced to progressive increase in the production and using of drug plants and natural medicines. Thus, care about quality of these products should also increase, since that incidence of contaminations, falsifications and adulterations are frequent. Consequently, these factors make difficult the safe practice of the phytotherapy due several factors, as the difficulty in the correct identification of the botanical material and the small amount or inexistence of studies of safety, effectiveness and quality of great number of the used plants. The maintenance and the warranty of the quality of these products involve a series of experiments that constitute the quality control, in which physical- chemistry analyses are inserted and by this are suggested in the present work. They are: preliminary phytochemical analysis, loss on drying (LD), determination of extractives (DE), total ashes (TA) and the chromatography analysis. Analyzed plant species were: Cinara scolymus (LD= 11.28% ± 0.29; DE= 35.42% ± 1.07; TA = 13.72% ± 1.89; Rf = 0.35 for cafeic acid and 0.58 for clorogenic acid), Peumus boldus (LD= 10.42% ± 0.30; DE= 31.36% ± 0.65; TA = 14.5% ± 0.26; Rf = 0.51 for boldine and 0.59 for yohimbine), Trichilia catigua (LD= 10.42% 0.08±; DE= 24.62% ± 0.74; TA = 6.90% ± 0.06; Rf = 0.58 for cinchonain), Maytenus ilicifolia (LD= 10.66% ± 0.28; DE= 30.26% ± 0.79; TA = 8.12% ± 0.14; Rf = 0.77 for epicatechin), Foeniculum vulgare (LD= 18.18% ± 0.37; DE= 24.01% ± 0.46; TA = 9.15% ± 0.29; Rf = 0.35 for cafeic acid), Mikania glomerata (LD= 10.57% ± 0.24; DE= 24.43% ± 0.60; TA = 5.40% ± 0.11; Rf = 0.79 for cumarin and 0.39 for o-cumaric acid), Hamamelis virginiana (LD= 6.6% ± 0.13; DE=% ±; TA = 4.75% ± 0.97; Rf = 0.18 for gallic acid), Hipericum perforatum (LD= 11.96% ± 0.24; DE= 23.31% ± 0.66; TA = 3.33% ± 0.08; Rf = 0.20 for hyperoside), Croton moritibensis (LD= 10.25% ± 0.08; DE= 2.29% ± 0.08; TA = 2.09% ± 0.07; Rf = 0.76 for lupeol), Achyrocline satureioides (LD= 10.02% ± 0.23; DE= 14.16% ± 0.25; TA = 3.17% ± 0.08; Rf = 0.35 for cafeic acid and 0.40 for quercetin), Passiflora alata (LD= 10.50% ± 0.25; DE= 21.02% ± 0.48; TA = 7.98% ± 0.16; Rf = 0.35 for cafeicacid and 040 for quercetin), Sambucus nigra (LD= 14.73% ± 0.34; DE= 29.69% ± 0.22; TA = 5.67% ± 0.15; Rf = 0.35 for cafeic acid and 0.36 for rutin), Plantago major (LD= 9.87% ± 0.19; DE= 42.70% ± 0.77; TA = 13.70% ± 0.37; Rf = 0.35 for cafeic acid and 0.58 for clorogenic acid), Arctostaphylos grape-ursi (LD= 9.49% ± 0.29; DE= 38.35% ± 0.78; TA = 3.23% ± 0.09; Rf= 0.49 for hydroquinone and 0.21 for gallic acid).
Keywords: quality control, physical-chemistry analyses, thin layer chromatography, drug plants
Dissertation PDF: Renato Braz
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